4 days old

Senior Scientist, Single Cell Sequencing

Pfizer
Cambridge, MA 02139
**ROLE SUMMARY**

We are seeking a motivated and dynamic individual to join the Molecular Profiling Group in Systems Immunology. This individual would support the development, validation, and implementation of single cell sequencing for drug-development in Inflammation and Immunology's portfolio areas. This person will execute technical and laboratory aspects of in-house single-cell analysis projects including the design and execution of laboratory experiments, evaluation of test data, as well the preparation of associated documentation and reports. This person must be technically able to do sample collection and single cell isolation, isolation of RNA or DNA extraction, standard Next Generation Sequencing (NGS) workflows and molecular biology associated with single-cell RNA sequencing. Meticulous attention to detail, proficient reasoning and problem-solving skills in experimental design are needed.



**ROLE RESPONSIBILITIES**



+ Supports the growth of single-cell analysis technology in drug development by supporting ongoing development, execution, validation and troubleshooting of single-cell assays, including single-cell RNA sequencing, single-cell epigenetic profiling, and antibody based single-cell analysis (CITEseq/REAPseq/TotalSeq/Abseq)

+ Performs operations of different single-cell analysis workflows; from tissue storage, sample preparation, library construction, data generation, data processing to interpretation of results, ensuring high level of quality and reliability of generated results.

+ Effective testing and optimization of tissue stabilization, dissociation, and purification protocols.

+ Effectively optimizing and troubleshooting of single-cell RNA sequencing library preparation.

+ Application of nucleic acid analysis techniques to monitor library construction quality control.

+ Implements data processing and analysis workflows, bioinformatics pipelines, and data storage procedures in collaboration with internal and external multidisciplinary teams.

+ Effectively utilizes available resources as needed to achieve timely execution of projects, works with cross-functional teams (internal and external) to execute plans and deliverables.

+ Serves as a subject matter resource for single-cell analysis technology, provides guidance on experimental design, method or platform selection, and data management to address a specific need.

+ Educates other scientists, provides hands-on training, and delivers internal and external presentations.

+ Learns various single-cell analysis platforms, stays on the top of the scientific and technical innovations, and follows new trends and developments in genomic technologies to uphold the department's competitive advantage.

+ Drafts experimental plans, validation reports, work instructions and SOPs.



**BASIC QUALIFICATIONS**



+ PhD in Biology or other related discipline with 0-3 years of experience or MS with 5+ years of relevant molecular and cellular biology experience

+ Extensive hands on molecular biology protocol and NGS technology expertise required

+ Must have at least 3 years of documented hands-on (wet lab) experience with NGS assay development, validation, troubleshooting, and reviewing of sample QC data OR tissue dissociation, cell isolation methods (e.g. FACS), cell culture and basic molecular biology (e.g. PCR, cloning).

+ Must have in-depth knowledge with different standard NGS platforms, bioinformatics pipelines and NGS data processing and interpretation solutions.

+ Must have experience in DNA/RNA extraction, nucleic acid quantitation and an understanding of quality control analysis.



**PREFERRED QUALIFICATIONS**



+ 1+ year of hands-on single-cell sequencing experience

+ Previous experience with tissue dissociation protocols, single-cell suspension generation required

+ Knowledge of single-cell RNA sequencing platforms (10x, inDrop, Drop-Seq, SMARTseq, Rhapsody, or other) greatly preferred.

+ Bioinformatics skills, including programing in R/python (or any other language), experience with command line, and familiarity with statistical software packages is highly desired.

+ Experience with computational and statistical approaches for high-dimensional data analysis is a plus.



**PHYSICAL/MENTAL REQUIREMENTS**



+ Ability to access and use a variety of computer software developed both in-house and off-the-shelf.

+ Occasional crouching, stooping, with frequent bending and twisting of upper body and neck.

+ Light to moderate lifting and carrying (or otherwise moves) objects including luggage and laptop computer with a maximum lift of 15-20 lbs.



\#LI-PFE



**Sunshine Act**

Pfizer reports payments and other transfers of value to health care providers as required by federal and state transparency laws and implementing regulations. These laws and regulations require Pfizer to provide government agencies with information such as a health care provider's name, address and the type of payments or other value received, generally for public disclosure. Subject to further legal review and statutory or regulatory clarification, which Pfizer intends to pursue, reimbursement of recruiting expenses for licensed physicians may constitute a reportable transfer of value under the federal transparency law commonly known as the Sunshine Act. Therefore, if you are a licensed physician who incurs recruiting expenses as a result of interviewing with Pfizer that we pay or reimburse, your name, address and the amount of payments made currently will be reported to the government. If you have questions regarding this matter, please do not hesitate to contact your Talent Acquisition representative.



**EEO & Employment Eligibility**

Pfizer is committed to equal opportunity in the terms and conditions of employment for all employees and job applicants without regard to race, color, religion, sex, sexual orientation, age, gender identity or gender expression, national origin, disability or veteran status. Pfizer also complies with all applicable national, state and local laws governing nondiscrimination in employment as well as work authorization and employment eligibility verification requirements of the Immigration and Nationality Act and IRCA. Pfizer is an E-Verify employer.



**Other Job Details:**



+ Eligible for Employee Referral Bonus



Pfizer is an equal opportunity employer and complies with all applicable equal employment opportunity legislation in each jurisdiction in which it operates.

Categories

Posted: 2019-11-08 Expires: 2019-12-08

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Senior Scientist, Single Cell Sequencing

Pfizer
Cambridge, MA 02139

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